| 產品編號 | bs-6033R |
| 英文名稱 | Rabbit Anti-Contactin 3 antibody |
| 中文名稱 | 腦源性免疫球蛋白超家族蛋白3抗體 |
| 別 名 | BIG 1; BIG-1; Contactin-3; Plasmacytoma-associated neuronal glycoprotein; Brain derived immunoglobulin superfamily protein 1; Brain-derived immunoglobulin superfamily protein 1; KIAA1496; PANG; PCS; CNTN3. |
| 研究領域 | 細胞生物 免疫學 神經生物學 細胞表面分子 腫瘤細胞生物標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Cow,Chicken,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 108kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Contactin 3: 301-400/1028 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Contactins mediate cell surface interactions during nervous system development. Contactin 3 has some neurite outgrowth-promoting activity. Function: Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity (By similarity). Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: In brain, it is expressed in frontal lobe, occipital lobe, cerebellum and amygdala. Similarity: Belongs to the immunoglobulin superfamily. Contactin family. Contains 4 fibronectin type-III domains. Contains 6 Ig-like C2-type (immunoglobulin-like) domains. SWISS: Q9P232 Gene ID: 5067 Database links: Entrez Gene: 5067 Human Entrez Gene: 18488 Mouse Omim: 601325 Human SwissProt: Q9P232 Human SwissProt: Q07409 Mouse Unigene: 12723 Human Unigene: 435479 Mouse Unigene: 10043 Rat |
| 產品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Contactin 3 Polyclonal Antibody, Unconjugated(bs-6033R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
