| 產品編號 | bs-5809R |
| 英文名稱 | Galectin 7 Rabbit pAb |
| 中文名稱 | 半乳糖凝集素7抗體 |
| 別 名 | Galectin7; Gal-7; GAL7; Galectin-7; HKL-14; Human keratinocyte lectin 14; Lectin galactoside binding soluble 7; LEG7_HUMAN; LGALS7; LGALS7B; p53-induced gene 1 protein; Pi7; PIG1; TP53I1. |
| 研究領域 | 腫瘤 細胞生物 免疫學 神經生物學 細胞凋亡 細胞粘附分子 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產品應用 | ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 15 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Galectin 7.: 31-100/136 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridization studies indicate that this lectin is specifically expressed in keratinocytes and found mainly in stratified squamous epithelium. A duplicate copy of this gene (GeneID:653499) is found adjacent to, but on the opposite strand on chromosome 19. [provided by RefSeq, Jul 2008]. Function: Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release. Subunit: Monomer. Subcellular Location: Cytoplasm. Nucleus. Secreted (Potential). Note=May be secreted by a non-classical secretory pathway. Tissue Specificity: Mainly expressed in stratified squamous epithelium. Similarity: Contains 1 galectin domain. SWISS: P47929 Gene ID: 653499 Database links: Entrez Gene: 3963 Human Entrez Gene: 653499 Human Entrez Gene: 16858 Mouse Omim: 600615 Human SwissProt: P47929 Human SwissProt: O54974 Mouse Unigene: 558355 Human Unigene: 707031 Human Unigene: 20973 Mouse Unigene: 6336 Rat |
| 產品圖片 |
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Galectin 7) polyclonal Antibody, Unconjugated (bs-5809R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
