| 產品編號 | bs-5711R |
| 英文名稱 | Rabbit Anti-CCDC106 antibody |
| 中文名稱 | 卷曲螺旋結構域蛋白106抗體 |
| 別 名 | Ccdc106; Coiled-coil domain-containing protein 106; Protein predicted by clone 23882; ZNF581; CC106_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 免疫學 細胞周期蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CCDC106: 201-280/280 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Promotes the degradation of p53/TP53 protein and inhibits its transactivity. Function: Promotes the degradation of p53/TP53 protein and inhibits its transactivity. Subunit: Interacts with p53/TP53. Subcellular Location: Nucleus. Note=Colocalizes with p53/TP53. SWISS: Q9BWC9 Gene ID: 29903 Database links: Entrez Gene: 29903 Human Entrez Gene: 232821 Mouse Omim: 613478 Human SwissProt: Q9BWC9 Human SwissProt: Q3ULM0 Mouse Unigene: 82482 Human Unigene: 482157 Mouse Unigene: 488775 Mouse CCDC106通過與p53蛋白相互作用，促進p53蛋白的降解，導致p53功能失活。CCDC106基因很可能是一個癌基因，CCDC106的高表達可能是導致具有野生型p53腫瘤發生的原因之一。 |
| 產品圖片 |
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- CCDC106 (bs-5711R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti- CCDC106 (bs-5711R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC106) Polyclonal Antibody, Unconjugated (bs-5711R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC106) Polyclonal Antibody, Unconjugated (bs-5711R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
