| 產品編號 | bs-5531R |
| 英文名稱 | phospho-PKMYT1 (Thr495) Rabbit pAb |
| 中文名稱 | 磷酸化蛋白激酶PKMYT1(Thr495)抗體 |
| 別 名 | PKMYT1(phospho T495); cdc2 inhibitory kinase; Membrane associated tyrosine and threonine specific cdc2 inhibitory kinase; Membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase; MYT1; Myt1 kinase; PKMYT 1; Pkmyt1; PMYT1_HUMAN; Protein kinase membrane associated tyrosine/threonine 1; Protein kinase Myt1; DKFZp547K1610; FLJ20093. |
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Specific References (2) | bs-5531R has been referenced in 2 publications.
[IF=6.714] Xu,et al.Ablation of PPARγ in subcutaneous fat exacerbates age-associated obesity and metabolic decline.(2018) Aging Cell. 17:. WB ; Mouse.
[IF=3.82] Xu,et al.Adipocytes affect castration-resistant prostate cancer cells to develop the resistance to cytotoxic action of NK cells with alterations of PD-L1/NKG2D ligand levels in tumor cells.(2018) The Prostate. 78:353-364. WB ; Human.
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| 產品類型 | 磷酸化抗體 |
| 研究領域 | 免疫學 信號轉導 細胞周期蛋白 轉錄調節因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human PKMYT1 around the phosphorylation site of Thr495: ED(p-T)LD |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] Function: Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development. Subunit: Interacts with CDC2-CCNB1 complex. Can also interact with PIN1 when phosphorylated by CDC2-CCNB1. Subcellular Location: Endoplasmic reticulum membrane. Golgi apparatus membrane. Post-translational modifications: Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. Contains 1 protein kinase domain. SWISS: Q99640 Gene ID: 9088 Database links: Entrez Gene: 9088 Human Entrez Gene: 268930 Mouse Omim: 602474 Human SwissProt: Q99640 Human SwissProt: Q9ESG9 Mouse Unigene: 77783 Human Unigene: 182193 Mouse Unigene: 204349 Rat |
| 產品圖片 |
Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: Molt-4 (Human) Cell Lysate at 30 ug
Lane 4: K562 (Human) Cell Lysate at 30 ug
Lane 5: U2os (Human) Cell Lysate at 30 ug
Lane 6: U251 (Human) Cell Lysate at 30 ug
Lane 7: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-phospho-PKMYT1 (Thr495) (bs-5531R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
