| 產品編號 | bs-5413R |
| 英文名稱 | phospho-MEK1 (Thr386) Rabbit pAb |
| 中文名稱 | 磷酸化絲裂原活化蛋白激酶1抗體 |
| 別 名 | MEK1(phospho Thr386); MEK1(phospho T386); p-MEK1(Thr386); p-MEK1(T386); Dual specificity mitogen activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAP/Erk kinase 1; Map2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEKK1; Mitogen activated protein kinase kinase 1; MKK 1; MKK1; PRKMK 1; PRKMK1; Protein kinase mitogen activated kinase 1(MAP kinase kinase 1); Protein kinase mitogen activated kinase 1; Protein kinase mitogen activated, kinase 1; MP2K1_HUMAN. |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 腫瘤 免疫學 信號轉導 轉錄調節因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Chicken,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human MEK1 around the phosphorylation site of Thr386: SP(p-T)PT |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Function: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. Subunit: Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3/ERK1 and RGS14 (By similarity). Forms an heterodimer with MAP2K2/MEK2 (By similarity). Forms heterodimers with KSR2 which further dimerize to form tetramers (By similarity). Interacts with ARBB2, LAMTOR3, MAPK1/ERK2, MORG1 and RAF1 (By similarity). Interacts with PPARG and with isoform 1 of VRK2. Interacts with Yersinia yopJ. Interacts with SGK1. Interacts with BIRC6/bruce. Subcellular Location: Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole body. Cytoplasm. Nucleus. Note=Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis. Tissue Specificity: Widely expressed, with extremely low levels in brain. Post-translational modifications: Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (RAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain. SWISS: P36507 Gene ID: 5605 Database links: Entrez Gene: 5604 Human Entrez Gene: 26395 Mouse Omim: 176872 Human SwissProt: Q02750 Human SwissProt: P31938 Mouse Unigene: 145442 Human Unigene: 248907 Mouse Unigene: 5850 Rat 絲裂原活化蛋白激酶是一組可以被多種細胞外信號即獲得蛋白絲/蘇氨酸激酶，處于胞漿信號傳導通路的終末位置，活化后轉位到核內，作用于核內轉錄因子，調節基因表達。它主要參與生長因子、激素、細胞因子、應激等各種刺激下細胞的反應、細胞的生長、分化過程。經研究證實，MAPK信號轉導通路存在于大多數細胞內，在將細胞外刺激信號轉導至細胞及其核內，并引起細胞生物學反應（如細胞增殖、分化、轉化及凋亡等）的過程中具有至關重要的作用。研究表明，MAPK信號轉導通路在細胞內具有生物進化的高度保守性，在低等原核細胞和高等哺乳類細胞內，目前均已發現存在著多條并行的MAPK信號通路，不同的細胞外刺激可使用不同的MAPK信號通路，通過其相互調控而介導不同的細胞生物學反應。 |
| 產品圖片 |
Sample:
placenta (Mouse) Lysate at 40 ug
Primary: Anti-phospho-MEK1 (Thr386) (Bs- 5413R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 48 kD
Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Adrenal gland Lysates
Lane 3: Rat Brain Lysates
Lane 4: Rat Adrenal gland Lysates
Lane 5: Human DU145 cell Lysates
Lane 6: Human HepG2 cell Lysates
Lane 7: Human A431 cell Lysates
Lane 8: Human HeLa cell Lysates
Primary: Anti-phospho-MEK1 (Thr386) (bs-5413R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43kDa
Observed band size: 50kDa
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MEK1 (Thr386)) Polyclonal Antibody, Unconjugated (bs-5413R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MEK1 (Thr386)) Polyclonal Antibody, Unconjugated (bs-5413R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
