| 產品編號 | bs-5382R |
| 英文名稱 | Phospho-NMDAR2B (Ser1480) Rabbit pAb |
| 中文名稱 | 磷酸化谷氨酸受體2B抗體 |
| 別 名 | NMDAR2B(phospho S1480); p-NMDAR2B(phospho S1480); NMDAR2B(phospho-Tyr1480); NMDAR2B(phospho-Y1480); p-NMDAR2B(Tyr1480); p-NMDAR2B(Y1480); AW490526; Glutamate [NMDA] receptor subunit epsilon 2; Glutamate [NMDA] receptor subunit epsilon-2; Glutamate Recepto |
 |
Specific References (3) | bs-5382R has been referenced in 3 publications.
[IF=6.208] Jiro Hasegawa Situmorang. et al. Ovariectomy Exacerbates Acute Ethanol-Induced Tachycardia: Role of Nitric Oxide and NMDA Receptors in the Rostral Ventrolateral Medulla. INT J MOL SCI. 2023 Jan;24(6):5087 IHC ; Rat.
[IF=2.47] Wen, Yuetao, et al. "Inhibition of Calcineurin A by FK506 Suppresses Seizures and Reduces the Expression of GluN2B in Membrane Fraction." Neurochemical Research (2017): 1-13. WB ; Rat.
[IF=0] Yang Q et al. Inhibition of Nwd1 activity attenuates neuronal hyperexcitability and GluN2B phosphorylation in the hippocampus. EBioMedicine. 2019 Sep;47:470-483. WB&IHC&IF ; Human&Mouse.
|
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 免疫學 神經生物學 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 163 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human NMDAR2B around the phosphorylation site of Ser1480: IE(p-S)DV |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq, Jul 2008]. Function: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Subunit: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of INADL, DLG4 and MAGI3 (By similarity). Interacts with HIP1 and NETO1. Interacts with DAPK1. Subcellular Location: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Post-translational modifications: Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity. Similarity: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. SWISS: Q13224 Gene ID: 2904 Database links: Entrez Gene: 2904 Human Entrez Gene: 14812 Mouse Omim: 138252 Human SwissProt: Q13224 Human SwissProt: Q01097 Mouse Unigene: 654430 Human Unigene: 436649 Mouse Unigene: 9711 Rat 谷氨酸受體（NR2B）是脊椎動物中樞神經系統興奮型神經傳遞的主要介質。在突觸可塑性極大腦學習及記憶功能方面起關鍵作用。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
