| 產品編號 | bs-0994P |
| 英文名稱 | SLT/SLT-IIe (O139) protein |
| 中文名稱 | 豬水腫素蛋白 |
| 別 名 | SLT/SLT-IIe(O139) immunoaffinty purified full length proten; Shiga-Like Toxin Type II Variant protein, Immuno affinity chromatography purified. |
| 理論分子量 | 35 kDa |
| 檢測分子量 | |
| 性 狀 | Lyophilized or Liquid |
| 物 種 | E.coli |
| 序 列 | Purified native protein |
| 內毒素 | Not analyzed |
| 活性 | Yes |
| 緩 沖 液 | 10 mM TBS (pH=7.4). |
| 保存條件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
Shiga-like toxin type II (SLT-II) and Shiga-like toxin type II variant (SLT-IIv) are cytotoxins produced by certain strains of Escherichia coli. Nucleotide sequence analyses had revealed that the structural genes for the A subunit and B subunit of SLT-II or SLT-IIv are arranged in an operon. Primer extension and S1 nuclease protection analyses identified a promoter for the slt-II operon 118 bases upstream of the slt-IIA gene. The slt-IIv promoter was demonstrated to be identical to the slt-II promoter. The slt-II and slt-IIv promoters differed significantly from the previously characterized Shiga toxin (stx) and Shiga-like toxin type 1 (slt-I) promoters. The transcriptional efficiencies of the stx and slt-II promoters were compared in fusions to the chloramphenicol acetyltransferase gene, and constitutive expression of the slt-II promoter was found to be equivalent to derepressed expression of the stx promoter. In contrast to the stx and slt-I promoters, the slt-II and slt-IIv promoters did not contain sequences for binding of the Fur repressor protein, and SLT-II production was not determined by iron levels in the media in various E. coli strains with wild-type or mutant ferric uptake regulation (fur) alleles. Northern (RNA) blot analysis demonstrated a single mRNA transcript for the slt-II operon, and further analysis of the slt-II operon by primer extension did not reveal an independent promoter for the B subunit gene. A putative rho-independent transcription terminator was identified 274 bases downstream of slt-IIB. These data indicated that the slt-II and slt-IIv operons differ from the stx/slt-I operon in regulation of their transcription by iron. Whether these regulatory differences enable the type I and type II groups of Shiga-like toxins to perform different roles in the pathogenesis of infectious diseases remains to be established. 致病性大腸肝菌-志賀樣毒素Ⅱ型變異體，又名豬水腫素（Shiga-Like Toxin Type II Variant），為病原性大腸肝菌的毒素-SLT-IIe屬于類志賀氏毒素家族成員。該毒素與其家族其他成員有著較高的同源性，SLT-IIe為VTEC(或SLTEC)所產生的一種蛋白質性質的細胞毒素，是引起人和動物出血性腹瀉和豬水腫病（仔豬、羊）的主要毒力因子。常見病原菌的血清型是O139。 豬水腫素主要引起豬水腫病又名豬胃腸水腫。是由病原性大腸肝菌的毒素引起斷奶仔豬的一種急性散發性疾病。主要表現為突然發病，運動共濟失調，驚厥，局部或全身麻痹及頭部水腫。剖檢變化為頭部皮下，胃壁及大腸間膜的水腫。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
