| 產品編號 | bs-5131R |
| 英文名稱 | Rabbit Anti-GLO1 antibody |
| 中文名稱 | 乙二醛酶1抗體 |
| 別 名 | Aldoketomutase; GLOD1; Glx I; GLYI; Glyoxalase I; Ketone aldehyde mutase; Lactoyl glutathione lyase; S D lactoylglutathione methylglyoxal lyase; LGUL_HUMAN; glo1; glyoxalase domain containing 1; Ketone-aldehyde mutase; Methylglyoxalase. |
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Specific References (1) | bs-5131R has been referenced in 1 publications.
[IF=4.49] Seyeon Oha et al. The attenuating effects of pyridoxamine on adipocyte hypertrophy and inflammation differ by adipocyte location. The Journal of Nutritional Biochemistry.2019 October, 72: 108173. WB&IHF ; Rat.
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| 研究領域 | 腫瘤 免疫學 糖尿病 內分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Pig,Cow,Dog,GuineaPig,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21kDa |
| 細胞定位 | 細胞核 細胞漿 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GLO1: 101-184/184 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The enzyme encoded by GLO1 is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. GLO is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. Subunit: Homodimer. Similarity: Belongs to the glyoxalase I family. SWISS: Q04760 Gene ID: 2739 Database links: Entrez Gene: 2739 Human Entrez Gene: 109801 Mouse Omim: 138750 Human SwissProt: Q04760 Human SwissProt: Q9CPU0 Mouse Unigene: 268849 Human Unigene: 261984 Mouse |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLO1) Polyclonal Antibody, Unconjugated (bs-5131R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
