| 產品編號 | bs-5103R |
| 英文名稱 | PTPMT1 Rabbit pAb |
| 中文名稱 | 線粒體蛋白酪氨酸磷酸酶1/DUSP23抗體 |
| 別 名 | DUSP23; FLJ46081; MOSP; NB4 apoptosis/differentiation related protein; Phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1; phosphoinositide lipid phosphatase; PLIP; PNAS 129; protein tyrosine phosphatase mitochondrial 1; Protein-tyrosine phosphatase mitochondrial 1; pten like phosphatase; PTEN-like phosphatase; PTPM1_HUMAN; Ptpmt1. |
| 研究領域 | 腫瘤 細胞生物 免疫學 轉錄調節因子 激酶和磷酸酶 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Pig,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PTPMT1: 101-201/201 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This protein phosphatase specifically mediates the dephosphorylation of mitochondrial proteins and consequently plays a central role in ATP production. It probably has a preference for proteins phosphorylated on Ser and/or Thr residues compared to phosphorylation on Tyr residues. It is likely to be involved in the regulation of insulin secretion in pancreatic beta cells. Function: Lipid phosphatase which dephosphorylates phosphatidylglycerophosphate (PGP) to phosphatidylglycerol (PG). PGP is an essential intermediate in the biosynthetic pathway of cardiolipin, a mitochondrial-specific phospholipid regulating the membrane integrity and activities of the organelle. Has also been shown to display phosphatase activity toward phosphoprotein substrates, specifically mediates dephosphorylation of mitochondrial proteins, thereby playing an essential role in ATP production. Has probably a preference for proteins phosphorylated on Ser and/or Thr residues compared to proteins phosphorylated on Tyr residues. Probably involved in regulation of insulin secretion in pancreatic beta cells (By similarity). Subcellular Location: Mitochondrion inner membrane. Note=Associated with the inner membrane. Similarity: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 tyrosine-protein phosphatase domain. SWISS: Q8WUK0 Gene ID: 114971 Database links: Entrez Gene: 114971 Human Omim: 609538 Human SwissProt: Q8WUK0 Human Unigene: 656205 Human Unigene: 728160 Human |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PTPMT1) Polyclonal Antibody, Unconjugated (bs-5103R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
