| 產品編號 | bs-5023R |
| 英文名稱 | Rabbit Anti-AGPAT1 antibody |
| 中文名稱 | 溶血磷脂酰基轉移酶抗體 |
| 別 名 | 1 AGPAT1; LPAAT alpha; LPAATA; MGC5423; 1 acyl sn glycerol 3 phosphate acyltransferase alpha; 1 acylglycerol 3 phosphate O acyltransferase 1 (acetoacetly Coenzyme A thiolase) ; 1 acylglycerol 3 phosphate O acyltransferase 1 (lysophosphatidic acid acyltransferase alpha); 1 acylglycerol 3 phosphate O acyltransferase 1; 1 AGP acyltransferase 1; AGPAT 1; G15 antibody Lysophosphatidic acid acyltransferase alpha; Lysophospholipid acyltransferase; MGC4007; OTTHUMP00000062693; Protein G15; Protein-G15. PLCA_HUMAN |
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Specific References (1) | bs-5023R has been referenced in 1 publications.
[IF=6.208] Chang-Hai Liu. et al. CircRNA-PI4KB Induces Hepatic Lipid Deposition in Non-Alcoholic Fatty Liver Disease by Transporting miRNA-122 to Extra-Hepatocytes. INT J MOL SCI. 2023 Jan;24(2):1297 WB ; Human.
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| 研究領域 | 腫瘤 細胞生物 免疫學 轉錄調節因子 糖尿病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AGPAT1: 101-200/283 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
AGPAT1 converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Function: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Subcellular Location: Membrane; Multi-pass membrane protein Similarity: Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. SWISS: Q99943 Gene ID: 10554 Database links: Entrez Gene: 10554 Human Omim: 603099 Human SwissProt: Q99943 Human Unigene: 409230 Human |
| 產品圖片 |
Sample:
Siver (Mouse) Lysate at 40 ug
Primary: Anti-AGPAT1 (bs-5023R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AGPAT1) Polyclonal Antibody, Unconjugated (bs-5023R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
