亚洲理论在线观看,中文字幕一区日韩精品,色一乱一伦一图一区二区精品,亚洲日本一区二区三区在线

掃碼關注公眾號           掃碼咨詢技術支持           掃碼咨詢技術服務
  
客服熱線：400-901-9800  客服QQ：4009019800  技術答疑  技術支持  質量反饋  人才招聘  關于我們  聯系我們
视频一区二区三区蜜桃麻豆,青青青爽在线视频观看,亚洲欧美成人一区二区在线电影
首頁 > 產品中心 > 一抗 > 產品信息
Rabbit Anti-KEAP1  antibody (bs-3648R)  
~~~促銷代碼KT202411~~~
訂購熱線：400-901-9800
訂購郵箱：sales@www.chomd.com.cn
訂購QQ：  400-901-9800
技術支持：techsupport@www.chomd.com.cn
說明書： 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包裝/詢價

產品編號 bs-3648R
英文名稱 Rabbit Anti-KEAP1  antibody
中文名稱 胞質接頭蛋白Keap1抗體
別    名 Cytosolic inhibitor of Nrf2; INrf 2; INrf2; Keap 1; KEAP1; KEAP1_HUMAN; Kelch like ECH associated protein 1; Kelch like protein 19; Kelch-like ECH-associated protein 1; Kelch-like protein 19; KIAA0132; KLHL 19; KLHL19; MGC10630; MGC1114; MGC20887; MGC4407; MGC9454.  
Specific References  (26)     |     bs-3648R has been referenced in 26 publications.
[IF=7.675] Xiaoxiao Zou. et al. Milk Fat Globule Membrane Relieves Fatigue via Regulation of Oxidative Stress and Gut Microbiota in BALB/c Mice. ANTIOXIDANTS-BASEL. 2023 Mar;12(3):712  WB ;  Mouse.  
[IF=6.291] Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-κB signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965  WB ;  Duck.  
[IF=5.81] Xinyue Chang. et al. The Protective Effect of Trichilia catigua A. Juss. on DEHP-Induced Reproductive System Damage in Male Mice. Front Pharmacol. 2022; 13: 832789  IHC ;  Mouse.  
[IF=5.552] Huang, Jianjia. et al. Lycium barbarum polysaccharides ameliorate canine acute liver injury by reducing oxidative stress, protecting mitochondrial function, and regulating metabolic pathways. J ZHEJIANG UNIV-SC B. 2023 Feb;24(2):157-171  WB ;  Dog.  
[IF=5.195] Yiyun Qian. et al. Seeds of Ginkgo biloba L. inhibit oxidative stress and inflammation induced by cigarette smoke in COPD rats through the Nrf2 pathway. J ETHNOPHARMACOL. 2023 Jan;301:115758  WB ;  Rat.  
[IF=4.868] Chen W et al. Obeticholic Acid Protects against Gestational Cholestasis-Induced Fetal Intrauterine Growth Restriction in Mice. Oxidative Medicine and Cellular Longevity, 2019, 1–17.  WB ;  Mouse.  
[IF=4.545] Yingran Liang. et al. Crocin ameliorates arsenic trioxide?induced cardiotoxicity via Keap1-Nrf2/HO-1 pathway: Reducing oxidative stress, inflammation, and apoptosis. Biomed Pharmacother. 2020 Nov;131:110713  WB ;  Rat.  
[IF=4.529] Shikuo Rong. et al. Amentoflavone Exerts Anti-Neuroinflammatory Effects by Inhibiting TLR4/MyD88/NF-κB and Activating Nrf2/HO-1 Pathway in Lipopolysaccharide-Induced BV2 Microglia. MEDIAT INFLAMM. 2022 Dec 06;2022:5184721  WB ;  Mouse.  
[IF=4.389] Junrong Zhang. et al. Neuroprotective effects of maize tetrapeptide-anchored gold nanoparticles in Alzheimer’s disease. Colloid Surface B. 2021 Apr;200:111584  WB ;  Mouse.  
[IF=4.36] Song Yang. et al. Ginseng root extract attenuates inflammation by inhibiting the MAPK/NF-κB signaling pathway and activating autophagy and p62-Nrf2-Keap1 signaling in vitro and in vivo. J Ethnopharmacol. 2021 Oct;:114739  WB ;  Mouse.  
[IF=4.19] Su, Chuanyang, et al. "Tetrachlorobenzoquinone activates Nrf2 signaling by Keap1 cross-linking and ubiquitin translocation, but not Keap1-Cullin3 complex dissociation." Chemical Research in Toxicology (2015).  WB ;  Human.  
[IF=4.109] Yingrong Ye. et al. Investigations into ferroptosis in methylmercury-induced acute kidney injury in mice. ENVIRON TOXICOL. 2023 Mar;:  WB ;  Mouse.  
[IF=4.105] Jacopo Di Gregorio. et al. UBXN7 cofactor of CRL3KEAP1 and CRL2VHL ubiquitin ligase complexes mediates reciprocal regulation of NRF2 and HIF-1α proteins. Bba-Mol Cell Res. 2021 Apr;1868:118963  WB ;  Human.  
[IF=3.905] Fen Xu. et al. Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway. Exp Cell Res. 2022 Mar;412:113025  IF ;  Human.  
[IF=3.83] Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653  WB ;  Human.  
[IF=3.743] Jin W et al. Tannic acid ameliorates arsenic trioxide-induced nephrotoxicity, contribution of NF-κB and Nrf2 pathways. Biomed Pharmacother. 2020 Jun;126:110047.  WB ;  rat.  
[IF=3.639] Huiling Mao. et al. Leucine protects bovine intestinal epithelial cells from hydrogen peroxide-induced apoptosis by alleviating oxidative damage. J SCI FOOD AGR. 2022 May 05  WB ;  Bovine.  
[IF=3.58] Su, Chuanyang, et al. "The electrophilic character of quinones is essential for the suppression of Bach1." Toxicology (2017).  
[IF=3.571] Zhao Y et al. Lycopene prevents DEHP-induced Leydig cell damage with the Nrf2 antioxidant signaling pathway in mice. J Agric Food Chem. 2019 Dec 8.  WB ;  Mouse.  
[IF=3.23] Chen, Ya-Hui, et al. "Caveolin-1 Expression Ameliorates Nephrotic Damage in a Rabbit Model of Cholesterol-Induced Hypercholesterolemia." PLOS ONE11.4 (2016): e0154210.  WB, IHC-P ;  Rabbit.  
[IF=3.205] Meng et al. Dietary resveratrol improves antioxidant status of sows and piglets and regulates antioxidant gene expression in placenta by Keap1-Nrf2 pathway and Sirt1. (2018) J.Anim.Sci.Biotechnol. 9:34  WB ;  Sow.  
[IF=3.11] Su, Chuanyang, et al. "Neohesperidin dihydrochalcone against CCl4-induced hepatic injury through different mechanisms The implication of free radical scavenging and Nrf2 activation." Journal of Agricultural and Food Chemistry(2015).  WB ;  Human.  
[IF=2.639] Jin Houet al. Silver Nanoparticles Induced Oxidative Stress and Mitochondrial Injuries Mediated Autophagy in HC11 Cells Through Akt/AMPK/mTOR Pathway. Biol Trace Elem Res . 2021 Mar;199(3):1062-1073.  WB ;  mouse.  
[IF=1.892] Kaiqiang Fu. et al. Tanshinone IIA exhibits anti-inflammatory and antioxidative effects in LPS-stimulated bovine endometrial epithelial cells by activating the Nrf2 signaling pathway. Res Vet Sci. 2021 May;136:220  WB ;  Bovine.  
[IF=1.719] Kacar S et al. Effect?of?acrylamide?on?BEAS-2B?normal?human?lung?cells:?Cytotoxic,?oxidative,?apoptotic?and?morphometric?analysis. Acta Histochem. 2019 Jul;121(5):595-603.  ICC ;  Human.  
[IF=1.641] Feng Gao. et al. Aucubin ameliorates the LPS‐induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF‐κB and activating the Keap1/Nrf2 signalling pathway. 2021 Apr 27  WB ;  Bovine.  
研究領域 腫瘤  細胞生物  免疫學  信號轉導  細胞凋亡  轉錄調節因子  激酶和磷酸酶  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 69kDa
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human KEAP1: 65-160/624 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008].

Function:
Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.

Subunit:
Homodimer. Forms a ternary complex with NFE2L2 and PGAM5. Interacts with the N-terminal regulatory domain of NFE2L2/NRF2. Interacts with BPTF and PTMA. Interacts with CUL3. Part of a complex that contains KEAP1, CUL3 and RBX1.

Subcellular Location:
Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus.

Tissue Specificity:
Broadly expressed, with highest levels in skeletal muscle.

Similarity:
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.

SWISS:
Q14145

Gene ID:
9817

Database links:

Entrez Gene: 9817 Human

Entrez Gene: 50868 Mouse

Entrez Gene: 100136900 Pig

Entrez Gene: 117519 Rat

Omim: 606016 Human

SwissProt: Q14145 Human

SwissProt: Q9Z2X8 Mouse

SwissProt: Q684M4 Pig

SwissProt: P57790 Rat

Unigene: 465870 Human

Unigene: 248266 Mouse

Unigene: 23467 Rat



Keap1是細胞抗氧化反應的重要調節蛋白，Keap1與Nrf2/ARE構成的信號通路在抗腫瘤、抗應激、調節GSH合成、抗凋亡、抗炎反應、神經保護等方面發揮著廣泛的細胞保護作用。
產品圖片
This image was generously provided by Yang Song, Ph.D. at Southwest University in Chong Qing, China. HepG2 cells were incubated with Rabbit Anti-KEAP1 Polyclonal Antibody (bs-3648R) at 4°C overnight and then mixed with Protein A agarose beads at 4°C for 3hrs. The solutions were centrifuged and the pellets were washed with lysis buffer, heated, and subsequently analyzed by Western blotting.
Sample: A673(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Primary: Anti-KEAP1 (bs-3648R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69 kD Observed band size: 69 kD
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-3648R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-3648R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-3648R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-3648R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-3648R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
版權所有 2004-2026 www.www.chomd.com.cn 北京博奧森生物技術有限公司
通過國際質量管理體系ISO 9001:2015 GB/T 19001-2016    證書編號: 00124Q34771R2M/1100
通過國際醫療器械-質量管理體系ISO 13485:2016 GB/T 42061-2022    證書編號: CQC24QY10047R0M/1100
京ICP備05066980號-1         京公網安備110107000727號