| 產品編號 | bs-2762R |
| 英文名稱 | Rabbit Anti-DARPP32 antibody |
| 中文名稱 | 多巴胺、cAMP調節磷蛋白抗體 |
| 別 名 | DARPP 32; DARPP-32; Dopamine and cAMP regulated neuronal phosphoprotein; Dopamine and cAMP regulated phosphoprotein DARPP 32; Dopamine and cAMP regulated phosphoprotein DARPP32; FLJ20940; IPPD; Neuronal phosphoprotein DARPP 32; PPP1R1B; Protein phosphatase 1 regulatory (inhibitor) subunit 1B; Protein phosphatase 1 regulatory subunit 1B. |
| 研究領域 | 神經生物學 信號轉導 通道蛋白 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat,GuineaPig (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DARPP32: 51-150/204 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]. Function: Inhibitor of protein-phosphatase 1. Subcellular Location: Cytoplasm. Post-translational modifications: Phosphorylation of Thr-34 is required for activity. Similarity: Belongs to the protein phosphatase inhibitor 1 family. SWISS: Q9UD71 Gene ID: 84152 Database links: Entrez Gene: 84152 Human Entrez Gene: 19049 Mouse Omim: 604399 Human SwissProt: Q9UD71 Human SwissProt: Q60829 Mouse Unigene: 286192 Human Unigene: 45372 Mouse Unigene: 70366 Rat ????DARPP-32通過自身不同位點的磷酸化對蛋白質磷酸化和去磷酸化過程發揮著雙向調控的作用.DARRP-32在腦內存在于接受多巴胺能投射的神經元中,其磷酸化過程受多種神經遞質的調控,在信號轉導途徑中處于中心位置,整合多種胞內信號轉導,調節神經元的電學和化學性質以及動物的生理行為反應,參與多種生理功能和病理過程,包括藥物成癮、抑郁癥、精神分裂癥等.此外,DARPP-32在非神經系統的功能也逐漸被人們所認識. ????多巴胺和cAMP調節的磷蛋白(dopamine and adenosine 3'5'-monophosphate-regulated phospho-protein,Mr 32 kD,DARPP-32)是一個具有雙重功能的獨特蛋白,它既是磷酸酶(如PP-1)的抑制劑,也是蛋白激酶(如PKA)的抑制劑。 |
| 產品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DARPP32 Polyclonal Antibody, Unconjugated(bs-2762R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
