| 產(chǎn)品編號(hào) | bs-3099R |
| 英文名稱 | Rabbit Anti-Phospho-Cofilin (Ser3) antibody |
| 中文名稱 | 磷酸化絲切蛋白(Ser3)抗體 |
| 別 名 | Cofilin (Phospho-Ser3); Cofilin (Phospho S3); 18 kDa phosphoprotein; CFL 1; CFL; CFL1; Cofilin 1; Cofilin 1 non muscle; Cofilin non muscle isoform; p18; COF1_HUMAN. |
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Specific References (3) | bs-3099R has been referenced in 3 publications.
[IF=4.014] Nuo Heng. et al. RhoA improves cryopreservation of rooster sperm through the Rho/RhoA-associated Kinase/cofilin pathway. POULTRY SCIENCE. 2022 Jul;:102051 WB ; Chicken.
[IF=2.894] Wufuer R et al. Downregulation of Rac1/PAK1/LIMK1/cofilin signaling pathway in colon cancer SW620 cells treated with Chlorin e6 photodynamic therapyPhotodiagnosis Photodyn Ther.2020 Dec 8;102143. WB ; Human.
[IF=2.705] Xue Y et al. Methamphetamine?reduces?expressions?of?tight?junction?proteins,?rearranges?F-actincytoskeleton?and?increases?the?blood?brain?barrier?permeability?via?the?RhoA/ROCK-dependent pathway. Biochem Biophys Res Commun. 2019 Feb 5;509(2):395-401. WB ; Rat.
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| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 生長(zhǎng)因子和激素 細(xì)胞粘附分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Sheep,Cow) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 18kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Cofilin around the phosphorylation site of Ser3: A(p-S)GV |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]. Function: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Subunit: Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods. Subcellular Location: Nucleus matrix. Cytoplasm, cytoskeleton. Tissue Specificity: Widely distributed in various tissues. Post-translational modifications: Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells. Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal. Similarity: Belongs to the actin-binding proteins ADF family. Contains 1 ADF-H domain. SWISS: P23528 Gene ID: 1072 Database links: Entrez Gene: 1072 Human Entrez Gene: 12631 Mouse Entrez Gene: 494432 Sheep Omim: 601442 Human SwissProt: P23528 Human SwissProt: P18760 Mouse SwissProt: Q6B7M7 Sheep Unigene: 52428 Cow Unigene: 170622 Human Unigene: 329655 Mouse Unigene: 11675 Rat Unigene: 13212 Sheep 絲切蛋白(cofilin)是細(xì)胞骨架塑形的重要調(diào)節(jié)因素,其主要功能是分解肌動(dòng)蛋白微絲和增加肌動(dòng)蛋白單體從肌動(dòng)蛋白微絲的末端解離的速度,從而促進(jìn)肌動(dòng)蛋白微絲的循環(huán),是目前為止已發(fā)現(xiàn)的肌動(dòng)蛋白結(jié)合蛋白家族中唯一能夠改變肌動(dòng)蛋白微絲扭曲結(jié)構(gòu)的蛋白. |
| 產(chǎn)品圖片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-Cofilin (Ser3) (bs-3099R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 18 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Cofilin (Ser3)) Polyclonal Antibody, Unconjugated (bs-3099R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Cofilin (Ser3)) Polyclonal Antibody, Unconjugated (bs-3099R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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