| 產品編號 | bs-2573R |
| 英文名稱 | Rabbit Anti-CD32 antibody |
| 中文名稱 | 低親和力免疫球蛋白γFc區(qū)受體II-a/Fc γ RII a抗體 |
| 別 名 | CD 32; CD32A; CD32B; CD32C; CDw32; Fc gamma RII; low affinity II receptor; Fc gamma receptor II; Fc-GAMMA-RII; Fc[g]RII; IgG Fc receptor II; FCG 2; FCG2; FcgammaRII; FCGR 2; FcGR; FCGR2; FCGR2A; FCGR2A1; FCGR2B; FCGR2C; FcgRII; Fcr-2; Fcr-3; FcRII a; FCRII; FcRII b; FcRII c; FcRIIC; IGFR 2; IGFR2; Ly-17; Ly-m20; LyM-1; Lymphocyte antigen 17; MGC23887; MGC30032; FCG2C_HUMAN. |
| 研究領域 | 細胞生物 免疫學 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000,Flow-Cyt=0.2μg/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31kDa |
| 檢測分子量 | 40 |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Fc gamma RII a: 201-300/317 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008] Function: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. Subunit: Isoform IIB1 interacts with measles virus N protein. N protein is released in the blood following lysis of measles infected cells. This interaction presumably block inflammatory immune response. Interacts with INPP5D/SHIP1. Interacts with FGR. Interacts with LYN. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells. Post-translational modifications: Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P12318 Gene ID: 2212 Database links: Entrez Gene: 2212 Human Entrez Gene: 2213 Human Entrez Gene: 9103 Human Omim: 146790 Human Omim: 604590 Human Omim: 612169 Human SwissProt: P12318 Human SwissProt: P31994 Human SwissProt: P31995 Human Unigene: 352642 Human Unigene: 154415 Rat |
| 產品圖片 |
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-CD32? (bs-2573R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Sample: K562 Cell Lysate at 30 ug
Primary: Anti- CD32 (bs-2573R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 33 kD
Blank control: U937 (blue). Primary Antibody:Rabbit Anti-CD32 antibody(bs-2573R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-2573R, 0.2μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
