| 產品編號 | bs-1903R |
| 英文名稱 | Rabbit Anti-Stra8 antibody |
| 中文名稱 | 視黃酸激活基因8抗體 |
| 別 名 | Stimulated by retinoic acid Gene 8; STRA8_HUMAN; Stimulated by retinoic acid gene 8 protein homolog; STRA 8; STRA8. |
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Specific References (5) | bs-1903R has been referenced in 5 publications.
[IF=6.582] Changliang Hou. et al. Stella Regulates the Development of Female Germline Stem Cells by Modulating Chromatin Structure and DNA Methylation. INT J BIOL SCI. 2022; 18(7): 3006–3018 WB ; Mouse.
[IF=5.048] Yuling Tang. et al. Transcriptomic Profiling of Mouse Mast Cells upon Pathogenic Avian H5N1 and Pandemic H1N1 Influenza a Virus Infection. Viruses-Basel. 2022 Feb;14(2):292 WB ; Mouse.
[IF=4.221] Jinxia Zhai. et al. BDE-209 induce spermatocytes arrest at early-pachytene stage during meiotic prophase I in mice. Toxicology. 2022 Feb;467:153061 WB,IF ; Mouse.
[IF=2.959] Zhuang M et al. Reelin regulates male mouse reproductive capacity via the sertoli cells.(2018) J. Cell. Biochem. 2018 Oct 18 WB ; Mouse.
[IF=0] Wenhuan Chai. et al. Visualizing Cathepsin K-Cre Expression at the Single-Cell Level with GFP Reporters. JBMR Plus. 2022 Dec;:e10706 IF ; Mouse.
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| 研究領域 | 細胞生物 發育生物學 細胞凋亡 細胞周期蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Stra8: 201-300/330 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a retinoic acid-responsive protein. A homologous protein in mouse has been shown to be involved in the regulation of meiotic initiation in both spermatogenesis and oogenesis, though feature differences between the mouse and human proteins suggest that these homologs are not entirely functionally equivalent. It is thought that this gene may play a role in spermatogenesis in humans. [provided by RefSeq, Nov 2010] Function: Required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase Subcellular Location: Cytoplasm. Tissue Specificity: Expressed specifically in testis. Post-translational modifications: Phosphorylated. SWISS: Q7Z7C7 Gene ID: 346673 Database links: Entrez Gene: 346673 Human Entrez Gene: 20899 Mouse Omim: 609987 Human SwissProt: Q7Z7C7 Human SwissProt: P70278 Mouse Unigene: 592279 Human Stra8是哺乳動物生殖細胞由有絲分裂轉變為減數分裂前特異表達的基因，是一種生殖細胞特異性表達并受視黃酸誘導的基因。Stra8是減數分裂起始的調節蛋白,參與了雌性胚胎期和雄性出生后生殖細胞的減數分裂起始過程。 |
| 產品圖片 |
Sample:
Testis (Rat) Lysate at 40 ug
Primary: Anti-bs-1903R (bs-1903R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-bs-1903R (bs-1903R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Stra8) Polyclonal Antibody, Unconjugated (bs-1903R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
