| 產品編號 | bs-1796R |
| 英文名稱 | Rabbit Anti-GLK antibody |
| 中文名稱 | 葡萄糖激酶抗體 |
| 別 名 | Glucokinase; ATP D hexose 6 phosphotransferase; GCK; GK; Glucokinase isoform 1; Hexokinase 4; Hexokinase D; Hexokinase D pancreatic isozyme; Hexokinase type IV; Hexokinase4; HexokinaseD; HHF 3; HHF3; HK 4; HK IV; HK4; HXKP; Maturity onset diabetes of the young 2; MODY 2; MODY2; GLUKA; RNGK2; HXK4_HUMAN. |
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Specific References (4) | bs-1796R has been referenced in 4 publications.
[IF=4.784] Pan Y et al. Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats. Int J Biol Macromol. 2019 Nov 8. pii: S0141-8130(19)37563-4. WB ; Rat.
[IF=4.414] Pasula, Madhu Babu. et al. Sex Dimorphic Glucose Transporter-2 Regulation of Hypothalamic Astrocyte Glucose and Energy Sensor Expression and Glycogen Metabolism. NEUROCHEM RES. 2022 Sep;:1-14 WB ; Rat.
[IF=3.483] Qiu F et al. Metabolic effects of mulberry branch bark powder on diabetic mice based on GC-MS metabolomics approach. Nutr Metab (Lond). 2019 Jan 31;16:10. WB ; Mouse.
[IF=2.43] Wang, Ping, et al. "Effects of a Novel Glucokinase Activator, HMS5552, on Glucose Metabolism in a Rat Model of Type 2 Diabetes Mellitus." Journal of Diabetes Research 2017 (2017). WB, IHC-P ; Rat.
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| 研究領域 | 免疫學 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Pig,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,Flow-Cyt=3ug/Test,ICC/IF=1:100,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GCK: 101-200/465 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Hexokinases phosphorylate glucose to produce glucose 6 phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of this gene results in three tissue specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose 6 phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non insulin dependent diabetes mellitus, also called maturity onset diabetes of the young, type 2; mutations have also been associated with persistent hyperinsulinemic hypoglycemia of infancy (PHHI). Function: Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage. Subunit: Monomer. Tissue Specificity: Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver. DISEASE: Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) [MIM:125851]; also shortened MODY-2. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Defects in GCK are the cause of familial hyperinsulinemic hypoglycemia type 3 (HHF3) [MIM:602485]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur. Similarity: Belongs to the hexokinase family. SWISS: P35557 Gene ID: 2645 Database links: Entrez Gene: 2645 Human Entrez Gene: 103988 Mouse Omim: 138079 Human SwissProt: P35557 Human SwissProt: P52792 Mouse Unigene: 1270 Human Unigene: 220358 Mouse Unigene: 10447 Rat |
| 產品圖片 |
Sample:
Panc-1(Human) Cell Lysate at 30 ug
Primary: Anti-GLK (bs-1796R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Protein: rat liver lysates, 30ug;
Primary: Anti-GLK(bs-1796R) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
ECL excitated the fluorescence;
Predicted band size : 51kD
Observed band size : 51kD
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GLK) polyclonal Antibody, Unconjugated (bs-1796R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-GLK antibody (bs-1796R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
