| 產品編號 | bs-1727R |
| 英文名稱 | Rabbit Anti-ABCG4 antibody |
| 中文名稱 | ABC膜轉運蛋白抗體 |
| 別 名 | ATP binding cassette, subfamily G member 4; ABCG 4; ATP binding cassette subfamily G member 4; WHITE 2; WHITE2; ABCG4_HUMAN. |
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Specific References (2) | bs-1727R has been referenced in 2 publications.
[IF=3.05] Matsumoto, Koichi, et al. "Immunohistochemical analysis of transporters related to clearance of amyloid-β peptides through blood–cerebrospinal fluid barrier in human brain." Histochemistry and Cell Biology (2015): 1-15. IHC-P ; Human.
[IF=1.22] Ueno, Masaki, et al. "Blood–brain barrier and blood–cerebrospinal fluid barrier in normal and pathological conditions." Brain Tumor Pathology (2016): 1-8. IHC-P ; Human.
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| 研究領域 | 免疫學 神經生物學 轉運蛋白 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Pig,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 71kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ABCG4: 551-646/646 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
he ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes. ABCG4 protein is highly expressed in both human and mouse brain, it is a 646aa molecule in human (chr 11q23) and mouse. It is an integral membrane protein may be involved in macrophage lipid homeostasis. The ABCG4 protein’s abundant expression in brain and close evolutionary relationship to the other members of the subfamily suggests a potential role in cholesterol transport. Function: May be involved in macrophage lipid homeostasis. Subcellular Location: Membrane; Multi-pass membrane protein (Probable). Similarity: Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. Contains 1 ABC transmembrane type-2 domain. Contains 1 ABC transporter domain. SWISS: Q9H172 Gene ID: 64137 Database links: Entrez Gene: 64137 Human Entrez Gene: 192663 Mouse Omim: 607784 Human SwissProt: Q9H172 Human Unigene: 126378 Human Unigene: 101876 Mouse Unigene: 29008 Rat |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ABCG4) Polyclonal Antibody, Unconjugated (bs-1727R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
