| 產品編號 | bs-1686R |
| 英文名稱 | Cyclin F Rabbit pAb |
| 中文名稱 | 周期素F抗體 |
| 別 名 | CCNF; F box only protein 1; FBX1; FBXO1; G2 mitotic specific cyclin F; CCNF_HUMAN. |
| 研究領域 | 細胞周期蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 86 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cyclin F: 451-550/786 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Cyclin F belongs to the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. Such proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Function: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication. Subunit: Component of the SCF(CCNF) complex consisting of CUL1, RBX1, SKP1 and CCNF. Interacts with CCNB1; interaction is required for nuclear localization of CCNB1. Interacts with CCP110; this nteraction leads to CCP110 ubiquitination and degradation via the proteasome pathway. Subcellular Location: Nucleus. Cytoplasm, cytoskeleton, centrosome, centriole. Note=Localization in the centrosome is rare in S phase cells and increases in G2 cells, Localizes on both the mother and daughter centrioles. Localization to centrosomes is not dependent on CP110. Also localizes to the nucleus. Tissue Specificity: Widely expressed. Post-translational modifications: Degraded when the spindle assembly checkpoint is activated during the G2-M transition. Degradation is not dependent on the proteasome or ubiquitin and depends on the C-terminal PEST sequence. Phosphorylated just before cells enter into mitosis. Similarity: Belongs to the cyclin family. Cyclin AB subfamily. Contains 1 cyclin N-terminal domain. Contains 1 F-box domain. SWISS: P41002 Gene ID: 899 Database links: Entrez Gene: 899 Human Entrez Gene: 12449 Mouse Omim: 600227 Human SwissProt: P41002 Human SwissProt: P51944 Mouse Unigene: 1973 Human Unigene: 77695 Mouse Cyclin F屬于Fbxs蛋白亞型的一種，表達在不同的細胞增殖周期進程中,主要表達在G2-M。 |
| 產品圖片 |
Sample:
U-2OS(Human) Cell Lysate at 30 ug
Primary: Anti- Cyclin F (bs-1686R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 86 kD
Observed band size: 86 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
