| 產品編號 | bs-0075R |
| 英文名稱 | Nociceptin Rabbit pAb |
| 中文名稱 | 孤菲肽/痛敏肽抗體 |
| 別 名 | PNOC; N/OFQ; N23K; N23K/N27K; Nociceptin, included; Npnc1; OFQ; OFQ/N; Orphanin FQ; PPNOC; prepronociceptin; Propronociceptin; PNOC_HUMAN. |
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Specific References (1) | bs-0075R has been referenced in 1 publications.
[IF=1.931] Lyu J et al. Botulinum toxin A alleviates orofacial nociception induced by orthodontic tooth movement through nociceptin/orphanin-FQ pathway in rats. Arch Oral Biol
. 2020 Sep;117:104817. WB ; Rat.
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| 研究領域 | 腫瘤 心血管 神經生物學 信號轉導 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 20 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nociceptin: 130-146/176 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Nociceptin (PNOC) is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development. Expressed predominantly in the spinal cord and brain, being more abundant in the hypothalamus and striatum. Also found in small amounts in ovary. Belongs to the opioid neuropeptide precursor family. Function: Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development (By similarity). Subcellular Location: Secreted. Tissue Specificity: Predominantly in the brain and spinal cord. Similarity: Belongs to the opioid neuropeptide precursor family. SWISS: Q13519 Gene ID: 5368 Database links: Entrez Gene: 5368 Human Entrez Gene: 18155 Mouse Omim: 601459 Human SwissProt: Q13519 Human SwissProt: Q64387 Mouse Unigene: 88218 Human Unigene: 16347 Mouse Unigene: 486163 Mouse Unigene: 87935 Rat 孤菲肽（Nociceptin又稱為Orphanin FQ; PPNOC; ORL1）是新發現的一種神經肽，由17個氨基酸組成，又稱痛敏肽。消化道腫瘤病人的血漿中OFQ含量明顯高于對照組，尤以結腸癌、胃癌最為明顯。 |
| 產品圖片 |
Sample:
Brain(Rat) lysate at 30ug;
Liver(Rat) lysate at 30ug;
Primary: Anti-Nociceptin(bs-0075R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 20kD
Observed band size : 20kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nociceptin Polyclonal Antibody, Unconjugated(bs-0075R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
