| 產品編號 | bs-1152R |
| 英文名稱 | Rabbit Anti-ATP1B2 antibody |
| 中文名稱 | 鈉鉀ATP酶通道蛋白抗體 |
| 別 名 | AT1B2_HUMAN; Adhesion molecule in glia (AMOG); Sodium/potassium-dependent ATPase subunit beta-2; ATPase Na+/K+ transporting subunit beta 2; |
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Specific References (4) | bs-1152R has been referenced in 4 publications.
[IF=41.444] Sun, Zeguo. et al. LINE-1 promotes tumorigenicity and exacerbates tumor progression via stimulating metabolism reprogramming in non-small cell lung cancer. MOL CANCER. 2022 Dec;21(1):1-24 WB ; Human.
[IF=6.304] Shiman Zuo. et al. Establishment of a novel mesenchymal stem cell-based regimen for chronic myeloid leukemia differentiation therapy. Cell Death Dis. 2021 Feb;12(2):1-15 WB ; Human.
[IF=4.088] Yanhui Jia. et al. The deacetylation of Akt by SIRT1 inhibits inflammation in macrophages and protects against sepsis. EXP BIOL MED. ;(): WB ; Human,Mouse.
[IF=1.689] Wang HY et al. Occludin Endocytosis is Involved in Disruption of the Intestinal Epithelial Barrier in a Mouse Model of Alcoholic Steatohepatitis. J Dig Dis. 2019 Jul 12. WB ; Human.
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| 研究領域 | 通道蛋白 糖蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,GuineaPig,Horse) |
| 產品應用 | WB=1:500-2000,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 33kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ATP1b2: 201-290/290 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. [provided by RefSeq, Jul 2008] Function: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known. Subunit: Composed of three subunits: alpha (catalytic), beta and gamma. Subcellular Location: Membrane; Single-pass type II membrane protein. Similarity: Belongs to the X(+)/potassium ATPases subunit beta family. SWISS: P14415 Gene ID: 482 Database links: Entrez Gene: 482 Human Omim: 182331 Human SwissProt: P14415 Human Unigene: 643540 Human 通道蛋白（Channel Protein） 鈉鉀ATP酶是位于細胞膜上的一種糖蛋白,與ATP 的分解和細胞內外鈉、鉀離子的轉運密切相關,哺乳動物各種組織細胞的鈉鉀ATP 酶的免疫學特性基本相同。 |
| 產品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Human K562 cell lysates
Lane 3: Human SH-SY5Y cell lysates
Primary: Anti-ATPase Na+/ K+ beta 2 (bs-1152R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 60 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
