| 產品編號 | bs-1235R |
| 英文名稱 | Hyaluronidase-1 Rabbit pAb |
| 中文名稱 | 透明質酸酶/玻璃酸酶抗體 |
| 別 名 | Hyaluronidase-1; HYAL1_HUMAN; Hyal-1; Hyaluronoglucosaminidase-1; Lung carcinoma protein 1 (LuCa-1); LUCA1; EC:3.2.1.35. |
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Specific References (4) | bs-1235R has been referenced in 4 publications.
[IF=11.092] Juan Cen. et al. A Water-Soluble Quercetin Conjugate with Triple Targeting Exerts Neuron-Protective Effect on Cerebral Ischemia by Mitophagy Activation. ADV HEALTHC MATER. 2022 Sep;:2200817 IHC ; Rat.
[IF=10.723] Jia-xin Hao. et al. A seminal perspective on the role of chondroitin sulfate in biomineralization. CARBOHYD POLYM. 2023 Jun;310:120738 IF ; Rat.
[IF=10.652] Junjie Liu. et al. A specific “switch-on” type magnetic resonance nanoprobe with distance-dominate property for high-resolution imaging of tumors. Chem Eng J. 2021 Jan;404:126496 IHC ; Mouse.
[IF=4.879] Petra ?ádníková. et al. The Degradation of Hyaluronan in the Skin. Biomolecules. 2022 Feb;12(2):251 WB,IHC,IF ; Human.
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| 研究領域 | 腫瘤 細胞生物 免疫學 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat Haase: 271-400/449 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Hyaluronidase degrades hyaluronic acid, which is an important structural proteoglycan found in basement membranes and also extracellular matrices. There are six members of the hyaluronidase family. Hyaluronidase PH20 is a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane and plays a role in sperm penetration through the the hyaluronic acid-rich cumulus cell layer surrounding the oocyte. Abnormal expression of this gene has been implicated in degradation of basement membranes leading to tumor invasion and metastasis. Function: May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth. Subcellular Location: Secreted. Lysosome. Tissue Specificity: Highly expressed in liver, kidney, lung and skin. Similarity: Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain. SWISS: Q76HN1 Gene ID: 367166 Database links: Entrez Gene: 3373 Human Entrez Gene: 15586 Mouse Omim: 607071 Human SwissProt: Q12794 Human SwissProt: Q91ZJ9 Mouse Unigene: 75619 Human Unigene: 10305 Mouse Unigene: 475658 Mouse Unigene: 124438 Rat HAase是一種具有高度底物特異性的蛋白酶，使透明質酸產生低分子化作用的酶之總稱,可以特異地作用于透明質酸，通過裂解葡萄糖胺和葡萄糖醛酸的分子鍵而將透明質酸分解為二糖成分，引起透明質酸的解聚和降解，透明質酸分子從膠原細纖維間隙中釋放出來并重新分布，主要定位于細胞外基質影響細胞的增殖、分化及遷移,并在胚胎發(fā)育和腫瘤發(fā)生發(fā)展過程中發(fā)揮作用,因而它在胚胎發(fā)育、血管形成、免疫監(jiān)視以及腫瘤發(fā)生發(fā)展過程中起著重要作用。 |
| 產品圖片 |
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Hyaluronidase-1 Polyclonal Antibody, Unconjugated(bs-1235R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Hyaluronidase-1 Polyclonal Antibody, Unconjugated(bs-1235R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-Hyaluronidase-1 antibody (bs-1235R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 2μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
