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Rabbit Anti-GRM2  antibody (bs-1161R)  
~~~促銷代碼KT202411~~~
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產品編號 bs-1161R
英文名稱 Rabbit Anti-GRM2  antibody
中文名稱 促代謝型谷氨酸受體2抗體
別    名 Metabotropic Glutamate Receptor 2; Glutamate Receptor Metabotropic 2; Gprc1b; AMPA selective glutamate receptor 2; GLUR 2; GLUR2; GLURB; Glutamate receptor metabotropic 2; Metabotropic Glutamate Receptor 2; GPRC1B; GRM 2; mGlu 2; MGlu2; MGLUR 2; Mglur2; GRM2_HUMAN.  
研究領域 腫瘤  免疫學  信號轉導  細胞凋亡  G蛋白偶聯受體  G蛋白信號  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse,Rat (predicted: Cow,Chicken,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 96kDa
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GRM2: 801-872/872 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.

Function:
Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.

Subunit:
Interacts with GRASP.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Widely expressed in different regions of the adult brain as well as in fetal brain.

Similarity:
Belongs to the G-protein coupled receptor 3 family.

SWISS:
Q14416

Gene ID:
2912

Database links:

Entrez Gene: 2912 Human

Entrez Gene: 108068 Mouse

Entrez Gene: 24415 Rat

Omim: 604099 Human

SwissProt: Q14416 Human

SwissProt: Q14BI2 Mouse

SwissProt: P31421 Rat

Unigene: 121510 Human

Unigene: 410822 Mouse

Unigene: 424639 Mouse

Unigene: 9681 Rat



谷氨酸受體-代謝能2;主要參與細胞信號轉導及突觸傳遞,在G蛋白偶聯受體蛋白信號轉導途徑中有一定的作用,并且有負調控腺苷酸環化酶的作用,為膜性蛋白,在胞膜、胞漿中表達。
產品圖片
Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Human SH-SY5Y cell lysates Primary: Anti-GRM2 (bs-1161R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 96 kD Observed band size: 120 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GRM2/GLUR2 Polyclonal Antibody, Unconjugated(bs-1161R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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