| 產品編號 | bs-0119R |
| 英文名稱 | FLIP Rabbit pAb |
| 中文名稱 | FLIP抗體 |
| 別 名 | CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p43; Flice-like Inhibitory protein; c FLIP; c FLIPL; c FLIPR; c FLIPS; c-FLIP; CASH; CASP8 and FADD like apoptosis regulator; CASP8 and FADD like apoptosis regulator precursor; CASP8AP1; Caspase Eight Related Protein; Caspase homolog; Caspase Homologue; Caspase Like Apoptosis Regulatory Protein; Caspase related inducer of apoptosis; CASPER; Cellular FLICE like inhibitory protein; CFLA; CFLAR; CLARP; FADD like anti apoptotic molecule; FADD Like Anti-apoptotic Molecule 1; FADD-like antiapoptotic molecule 1; FADD like antiapoptotic molecule 1; FADD Like Apoptosis Regulator; FLAME 1; FLAME; FLAME1; FLAME-1; FLICE Inhibitor Protein; FLIP; FLIPs; I FLICE; I-FLICE; Inhibitor of FLICE; Inhibitor of FLICE; MACH Related Inducer of Toxicity; MACH-related inducer of toxicity; mFLIP; MRIT; USURPIN; Usurpin beta; FLICE-like inhibitory protein short form; FLICE-like inhibitory protein long form; CFLAR_HUMAN; Cellular FLICE-like inhibitory protein. |
| 研究領域 | 腫瘤 細胞生物 信號轉導 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43/52 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CASP8 and FADD-like apoptosis regulator subunit p43: 7-100/480 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011] Function: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. Subunit: TNFRSF6 stimulation triggers recruitment to the death-inducing signaling complex (DISC) formed by TNFRSF6, FADD and caspase-8. A proteolytic fragment (p43) stays associated with the DISC. Also interacts with caspase-10, caspase-3, TRAF1, TRAF2 and Bcl-X(L) (in vitro). Interacts with HBV protein X. Tissue Specificity: Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. Post-translational modifications: Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12. Similarity: Belongs to the peptidase C14A family. Contains 2 DED (death effector) domains. SWISS: O15519 Gene ID: 8837 Database links: Entrez Gene: 8837 Human Entrez Gene: 12633 Mouse Omim: 603599 Human SwissProt: O15519 Human SwissProt: O35732 Mouse Unigene: 390736 Human Unigene: 336848 Mouse FLIP參與凋亡的調節。此抗體在長型和短型的FLIP異構體中均表達。短型FLIP包含2個死亡效應基因結構區，同源于FAS相關蛋白死亡效應基因結構區。長型FLIP包含1個附加的Caspase 樣結構區，但是他缺少一個催化部位和在大多數Caspase 蛋白中形成底物結合束的殘基。 |
| 產品圖片 |
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-FLIP (bs-0119R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43/52 kD
Observed band size: 52 kD
Sample:
Muscle(Mouse) Lysate at 40 ug
Primary: Anti-FLIP (bs-0119R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43/52 kD
Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FLIP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FLIP/c FLIP Polyclonal Antibody, Unconjugated(bs-0119R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (Black line): HUVEC (Black).
Primary Antibody (green line): Rabbit Anti-FLIP antibody (bs-0119R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-FLIP antibody (bs-0119R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
