| 產(chǎn)品編號(hào) | bs-0155R |
| 英文名稱 | CRP Rabbit pAb |
| 中文名稱 | C-反應(yīng)蛋白抗體 |
| 別 名 | C-reactive protein; C Reactive Protein; C reactive protein pentraxin related; CRP; MGC88244; PTX 1; PTX1. C反應(yīng)蛋白 |
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Specific References (8) | bs-0155R has been referenced in 8 publications.
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| 研究領(lǐng)域 | 心血管 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 生長(zhǎng)因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CRP: 125-224/224 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Pentraxins are plasma proteins characterized by their pentameric discoid assembly and their Ca2+ dependent ligand binding, such as Serum amyloid P component (SAP) and C-reactive Protein (CRP), which are cytokine-inducible acute-phase proteins implicated in innate immunity. CRP binds to ligands containing phosphocholine, SAP binds to amyloid fibrils, DNA, chromatin, fibronectin, C4-binding proteins and glycosaminoglycans. "Long" pentraxins have N-terminal extensions to the common pentraxin domain; one group, the neuronal pentraxins, may be involved in synapse formation and remodeling, and they may also be able to form heteromultimers. Function: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Subunit: Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits. Subcellular Location: Secreted. Tissue Specificity: Found in plasma. Similarity: Belongs to the pentaxin family. Contains 1 pentaxin domain. SWISS: P02741 Gene ID: 1401 Database links: Entrez Gene: 1401 Human Omim: 123260 Human SwissProt: P02741 Human Unigene: 76452 Human C反應(yīng)蛋白 CRP（C-reactive protin）是與C性肺炎球菌的多糖起反應(yīng)的一種蛋白，在某些細(xì)菌的感染過程中出現(xiàn)在血漿中。 CRP是在一些病理性情況下出現(xiàn)于病人血清中的一種糖蛋白，特別是在伴有肯炎癥和組織壞死疾病的急性期。血清中出現(xiàn)的這種異常蛋白能和肺炎C-多糖體發(fā)生沉淀反應(yīng)，當(dāng)疾病好轉(zhuǎn)或緩解時(shí)，CRP減低或消失。 臨床意義: (1)各種急性化膿性炎癥、菌血癥、組織壞死(心肌梗塞、手術(shù)、嚴(yán)重創(chuàng)傷、燒傷等)、惡性腫瘤、結(jié)締組織病、腎移殖后急性排異反應(yīng)、急性風(fēng)濕熱、風(fēng)濕性關(guān)節(jié)炎、類風(fēng)濕性關(guān)節(jié)炎、心肌炎急性期、肺炎、肺結(jié)核、系統(tǒng)性紅斑狼瘡、急性肝炎和闌尾炎等，血清中CRP含量可增高，它的出現(xiàn)比其它急性期的反應(yīng)物質(zhì)早，所以對(duì)疾病的早期診斷很有幫助。 CRP可以鑒別細(xì)菌炎癥或者是病毒性炎癥。細(xì)菌性炎癥CRP可以明顯增高，而病毒性炎癥CRP的血清含量一般都在正常范圍。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRP) Polyclonal Antibody, Unconjugated (bs-0155R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CRP Polyclonal Antibody, Unconjugated(bs-0155R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CRP Polyclonal Antibody, Unconjugated(bs-0155R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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