| 產品編號 | bs-0275R |
| 英文名稱 | Bassoon Rabbit pAb |
| 中文名稱 | 鋅指蛋白231抗體 |
| 別 名 | Bassoon; BSN; KIAA0434; Neuronal double zinc finger protein; Presynaptic cytomatrix protein; Protein bassoon; Zinc finger protein 231; ZNF 231; ZNF231; Neuronal double zinc finger protein; Zinc-finger protein 231; BSN_HUMAN; ZNF 231. |
| 研究領域 | 腫瘤 免疫學 鋅指蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 432 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BSN: 2901-3000/3926 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Neurotransmitters are released from a specific site in the axon terminal called the active zone, which is composed of synaptic vesicles and a meshwork of cytoskeleton underlying the plasma membrane. The protein encoded by this gene is thought to be a scaffolding protein involved in organizing the presynaptic cytoskeleton. The gene is expressed primarily in neurons in the brain. A similar gene product in rodents is concentrated in the active zone of axon terminals and tightly associated with cytoskeletal structures, and is essential for regulating neurotransmitter release from a subset of synapses. [provided by RefSeq, Jul 2008]. Function: Is thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act through binding to ERC2/CAST1. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses. Subunit: Interacts with ERC2/CAST1, RIMS1 and UNC13A. Part of a complex consisting of ERC2, RIMS1 and BSN. Subcellular Location: Cytoplasm. Cell junction, synapse, synaptosome. Cytoplasm, cytoskeleton. Note=Localized to the active zone of presynaptic density. Tissue Specificity: Exclusively expressed in brain. Post-translational modifications: Myristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization. SWISS: Q9UPA5 Gene ID: 8927 Database links: Entrez Gene: 8927 Human Entrez Gene: 12217 Mouse Omim: 604020 Human SwissProt: Q9UPA5 Human SwissProt: O88737 Mouse Unigene: 194684 Human Unigene: 20425 Mouse Unigene: 29999 Rat 鋅指蛋白是80年代中期發現的一類DNA結合蛋白，在真核生物中,鋅指蛋白可能是最大的一類DNA結合蛋白，并且由鋅指蛋白調控基因表達是發育和其他過程的一個非常普遍的現象。在最近的幾年中，發現某些鋅指蛋白對肌肉的發育具有重要的調節作用。 |
| 產品圖片 |
Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Bassoon Polyclonal Antibody, Unconjugated(bs-0275R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
