| 產品編號 | bs-0537R |
| 英文名稱 | ANGPTL2 Rabbit pAb |
| 中文名稱 | 血管生成素樣蛋白2抗體 |
| 別 名 | ANGPTL2; Angiopoietin like 2; Angiopoietin related protein 2; Angiopoietin-like protein 2; Angiopoietin-related protein 2; ANGL2_HUMAN; Angptl2; ANGL2; Arp2; HARP; MGC8889. |
| 研究領域 | 腫瘤 心血管 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ANGPTL2: 401-493/493 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
ANGPTL2/ANG-2 (Angiopoietin-like Protein 2) Induces sprouting in endothelial cells through an autocrine and paracrine action. [Subcellular location] Secreted. [Tissue specificity] Widely expressed in heart, small intestine, spleen and stomach. Also found in lower levels in colon, ovary, adrenal gland, skeletal muscle and in prostate. [PTM] N-glycosylated. [Similarity] Contains 1 fibrinogen C-terminal domain. ANGPTL2/ANG-2 (Angiopoietin-like Protein 2). Function: Induces sprouting in endothelial cells through an autocrine and paracrine action. Subcellular Location: Secreted. Tissue Specificity: Widely expressed in heart, small intestine, spleen and stomach. Also found in lower levels in colon, ovary, adrenal gland, skeletal muscle and in prostate. Similarity: Contains 1 fibrinogen C-terminal domain. SWISS: Q9UKU9 Gene ID: 23452 Database links: Entrez Gene: 23452 Human Entrez Gene: 26360 Mouse Omim: 605001 Human SwissProt: Q9UKU9 Human SwissProt: Q9R045 Mouse Unigene: 653262 Human Unigene: 208919 Mouse Unigene: 162660 Rat Ang1/2與血管生成關系最為密切,目前有關該家族成員在腫瘤血管生成中的研究正日益增多。近年來也用于糖尿病導致的血管損傷研究也是熱門課題. |
| 產品圖片 |
Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti-ANGPTL2 (bs-0537R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Paraformaldehyde-fixed, paraffin embedded (Human uterine muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angptl2) Polyclonal Antibody, Unconjugated (bs-0537R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
