| 產(chǎn)品編號 | bs-1025R |
| 英文名稱 | Rabbit Anti-HGF antibody |
| 中文名稱 | 肝細胞生長因子α抗體 |
| 別 名 | HGF alpha; HGFA; F TCF; Hepatocyte growth factor; Fibroblast derived tumor cytotoxic factor; Hepatocyte growth factor precursor; Hepatopoietin A; HPTA; Lung fibroblast derived mitogen; Scatter factor; SF; hepatocyte growth factor alpha-chain; Hepatocyte growth factor alpha chain; HGF_HUMAN. |
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Specific References (13) | bs-1025R has been referenced in 13 publications.
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| 研究領(lǐng)域 | 腫瘤 免疫學 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog,GuineaPig,Horse) |
| 產(chǎn)品應用 | WB=1:200-500,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HGF alpha: 201-300/723 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the lasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. Function: HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity. Subunit: Dimer of an alpha chain and a beta chain linked by a disulfide bond. DISEASE: Defects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39) [MIM:608265]. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Similarity: Belongs to the peptidase S1 family. Plasminogen subfamily. Contains 4 kringle domains. Contains 1 PAN domain. Contains 1 peptidase S1 domain. SWISS: P14210 Gene ID: 3082 Database links: Entrez Gene: 3082 Human Entrez Gene: 15234 Mouse Omim: 142409 Human SwissProt: P14210 Human SwissProt: Q08048 Mouse Unigene: 396530 Human Unigene: 267078 Mouse Unigene: 10468 Rat 肝細胞生長因子（HGF)是一種多肽生長因子，具有促進包括肝細胞、上皮細胞、內(nèi)皮細胞、造血細胞等多種類型細胞的生長、遷移和形態(tài)發(fā)生的作用. 它還參與多種細胞的增殖、遷移,對各類腫瘤的侵襲轉(zhuǎn)移有著重要的誘導作用. HGF除了由肝臟大量產(chǎn)生并釋放進入血液，還可從胚胎肺成纖維細胞株中分離得到，因此又稱擴散因子（scatter factor）或稱肺成纖維細胞衍生生長因子（fibroblast-derived growth factor）。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Rat Placenta tissue lysates
Lane 2: Rat Cerebellum tissue lysates
Primary: Anti-HGF (bs-1025R) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54/80 kDa
Observed band size: 60 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (HGF) Polyclonal Antibody, Unconjugated (bs-1025R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
